Part:BBa_K1020006:Design

YbhGFSR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1270
  Illegal BglII site found at 3160
  Illegal BglII site found at 4514
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2943
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3858
  

Design Notes

A complete sequence of ybhGFSR transporter and the native E.coli ybhG start codon being changed from GTG to ATG.

Source

E.coli K12